After an accident in the mysterious lab of maniacal scientist, Dr Simon Barsinister (Peter Dinklage), an ordinary beagle unexpectedly finds himself with unimaginable powers and the ability to speak. Armed with a fetching superhero costume, Underdog (voiced by comedian, Jason Lee) vows to protect the beleaguered citizens of Capitol City and, in particular, one beautiful spaniel named Polly Purebread (voiced by Amy Adams). When an evil plot by Barsinister and his overgrown henchman, Cad (Patrick Warburton) threatens to destroy Capitol City, only Underdog can save the day.